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Prospec/NNMT Mouse/Nicotinamide N-Methyltransferase Mouse Recombinant/2µg/ENZ-1037-2µg-2µg
Cataloguenumber
ENZ-1037
Synonyms
NicotinamideN-methyltransferase,Nnmt,icotinamideN-methyltransferaseisoform1.
Introduction
NNMTispartofthefamilyoftransferases,especiallythosetransferringone-carbongroupmethyltransferases.NNMTismostlyexpressedintheliver,andalowerexpressionisseeninthekidney,lung,skeletalmuscle,placentaandheart.NNMTcatalyzestheN-methylationofnicotinamideandotherpyridinestoformpyridiniumions.Thisactivityissignificantforbiotransformationofmanydrugsandxenobioticcompounds.NNMTisaccountablefortheenzymaticactivitywhichusesS-adenosylmethionineasthemethyldonor.NNMTexpressionisrelatedwithtumorstageandDFStimeinhepatocellularcarcinomacases.NNMTisagoodcandidateasatumorMarkerofvariouskindsofcancers.NNMTserumlevelshavesignificanceintheprematuredetectionandinthemanagementofpatientswithcolorectalcancer.
Description
NNMTMouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining288aminoacids(1-264a.a)andhavingamolecularmassof32.1kDa.NNMTisfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
NNMTproteinsolution(1mg/ml)containing20mMTris-HCl(pH8.0),10%glyceroland1mMDTT.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMESGFTSKDTYLSHFNPRDYLEKYYSFGSRHCAENEILRHLLKNLFKIFCLGAVKGELLIDIGSGPTIYQLLSACESFTEIIVSDYTDQNLWELQKWLKKEPGAFDWSPVVTYVCDLEGNRMKGPEKEEKLRRAIKQVLKCDVTQSQPLGGVSLPPADCLLSTLCLDAACPDLPAYRTALRNLGSLLKPGGFLVMVDALKSSYYMIGEQKFSSLPLGWETVRDAVEEAGYTIEQFEVISQNYSSTTSNNEGLFSLVGRKPGRSE.
SafetyDataSheet
SDS
