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Prospec/Welqut Protease/Welqut Protease Staphylococcus aureus Recombinant/1,000U/ENZ-1113-1,000U-1,000U
Cataloguenumber
ENZ-1113
Introduction
WELQutProteaseisanextremelyspecificandrecombinantserineproteasefromStaphylococcusaureus.TheWELQutProteaseidentifiesandaccuratelycleavesrecombinantproteinsthathasarecognitionsequenceaddedtothem,withtheaminoacidsequenceTrp,Glu,Leu,Gln,X(anyaminoacid).WELQutProteasecutexternallyfromtherecognitionsequence,therefordoesn’tleaveextraaminoacidsboundtothetargetprotein.Theproteaseisn’ttemperaturesensitive(worksin4-30°C)orpHsensitive(pH6.5-9.0),also,thereisnoneedinanyparticularbuffers.
Description
WelqutProteaseRecombinantisasingle,non-glycosylatedpolypeptidechaincontaining204aminoacidsandhavingamolecularmassof22kDa.TheWelqutProteaseispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
WelqutProteasecontains10mMNa2HPO4,50%glycerol,1.8mMKH2PO4,pH7.3,140mMNaCland2.7mMKCl.
StABIlity
Purity
Greaterthan97.0%asdeterminedbySDS-PAGE.
UnitDefinition
Eachunitisdefinedastheamountofenzymerequiredtocleave≥99%of100μgofacontrolproteinin16hat20°C.Enzymeactivityisassayedin100μl100mMTris-HCl(pH8.0).
SafetyDataSheet
SDS