
Cataloguenumber
ENZ-1040
Synonyms
Dualspecificityproteinphosphatase18,Lowmolecularweightdualspecificityphosphatase20,LMW-DSP20,DUSP18,LMWDSP20,VHP,DUSP26.
Introduction
Dualspecificityphosphatase18(DUSP18)belongstothedual-specificityphosphatase(DSP)family,whichcatalyzesdephosphorylationofphosphotyrosineandphosphothreonineresidues.DUSP18hasapreferentialenzymaticactivityforphosphorylatedtyrosineresiduesoverthreonineresidues,inadditionDUSP18dephosphorylatesp-nitrophenylphosphate(pNPP)invitro.FurThermore,DUSP18isinhibitedbyiodoarecticacidandisactivatedbymanganeseions.DUSP18isextensivelyexpressedwiththehighestlevelsintheliver,brain,ovaryandtestis.
Description
DUSP18producedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining212aminoacids(1-188a.a.)andhavingamolecularmassof23.6kDa.DUSP18isfusedtoa24aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheDUSP18proteinsolution(0.5mg/ml)isformulatedin20mMTris-HClbuffer(pH8.0),0.1mMPMSF,1mMDTT,40%glyceroland1mMEDTA.
StABIlity
Purity
BIOLOGicalActivity
Specificactivityis>300units/mg,andisdefinedastheamountofenzymethathydrolyze1.0nmoleofp-nitrophenylphosphate(pNPP)perminuteatpH7.5at37°C.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSHMTAPSCAFPVQFRQPSVSGLSQITKSLYISNGVAANNKLMLSSNQITMVINVSVEVVNTLYEDIQYMQVPVADSPNSRLCDFFDPIADHIHSVEMKQGRTLLHCAAGVSRSAALCLAYLMKYHAMSLLDAHTWTKSCRPIIRPNSGFWEQLIHYEFQLFGKNTVHMVSSPVGMIPDIYEKEVRLMIPL
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.