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Prospec/Carboxypeptidase B Rat/Carboxypeptidase-B Rat Recombinant/5mg/ENZ-475-5mg-5mg
Cataloguenumber
ENZ-475
Synonyms
Introduction
CarboxypeptidaseB(EC3.4.17.2)catalyzeshydrolysisofthebasicaminoacidslysine,arginineandornithinefromtheC-terminalendofpolypeptides.TheMwwasfoundtobe34.5kDa,optimunpH-7.9,andpI-6.CarboxypeptidaseBisinhibitedbyarginine,lysineandornithine.Theenzymeisnotinhibitedbydi-isopropylfluorophosphate(DFP),butitisinhibitedbymetalchelatingagents,e.g.,EDTA,1,10-phenanthroline.
Description
RecombinantRatCarboxypeptidase-BisexpressedinE.ColihavingaMwof31kDaispurifiedbystandardchromatographytechniques.RecombinantRatCarboxypeptidase-BisfreefromforeignenzymessuchascarboxypeptidaseA&chymotrypsin.RecombinantCarboxypeptidase-BisfreefromproteaseinhibitorssuchasPMSFandEDTA.
Source
PhysicalAppearance
Formulation
Theproteinwaslyophilizedwith100mMNaCl,mannitoland20mMTrispH-7.5.
Solubility
ItisrecommendedtoreconstitutethelyophilizedRatCarboxypeptidase-Binsterile18MΩ-cmH2Oor25mMTris-HClpH7.65notlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
StorethelyophilizedCarboxypeptidase-Bat4°C.Uponreconstitutetheproteinshouldbestoredat4°Cfor2weeksandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
UnitDefinition
BIOLOGicalActivity
170units/mgprotein.
SafetyDataSheet
SDS