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Prospec/NT5M Human/5',3'-Nucleotidase, Mitochondrial Human Recombinant/50µg/ENZ-160-50µg-50µg
Catalogue number
ENZ-160
Synonyms
5"(3")-deoxyribonucleotidase mitochondrial, 5",3"-nucleotidase, mitochondrial, Deoxy-5"-nucleotidase 2, dNT-2, NT5M, DNT2.
Introduction
NT5M is a member of the 5"(3")-deoxyribonucleotidase family. NT5M localizes to the mitochondrial matrix. The NT5M enzyme dephosphorylates the 5"- and 2"(3")-phosphates of uracil and thymine deoxyribonucleotides. The NT5M gene is located on chromosome 17 p11.2 in the critical region deleted in the Smith-Magenis syndrome (SMS), a genetic disease of unidentified etiology.
Description
NT5M Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (32-228 a.a.) and having a molecular mass of 25.1kDa.NT5M is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Source
Physical Appearance
Formulation
NT5M protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC.
Safety Data Sheet
SDS
