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Prospec/NT5C2 Human/5'-Nucleotidase Cytosolic II Human Recombinant/1mg/ENZ-173-1mg-1mg
Cataloguenumber
ENZ-173
Synonyms
Cytosolicpurine5"-nucleotidase,Cytosolic5"-nucleotidaseII,NT5C2,NT5B,NT5CP,PNT5,GMP,cN-II.
Introduction
NT5C2(NT5B/PNT5)hasavitalroleinthemaintenanceofpurine/pyrimidinenucleotides.NT5C2containsaphosphotransferaseactivesitewhichcatalyzesthedephosphorylationof6-hydroxypurinenucleoside5"-monophosphates.FurThermore,NT5C2regulatesthelevelofinosinemonophosphate(IMP)andguanosinemonophosphate(GMP)poolsinsidecellsbywayofhydrolysis.
Description
NT5C2HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining581aminoacids(1-561a.a.)andhavingamolecularmassof67.1kDa.NT5C2isfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
NT5C2proteinsolution(0.5mg/ml)containing20mMTris-HClbuffer(pH8.0),30%glycerol,0.1MNaCl,1mMDTTand0.1mMPMSF.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEEE.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.