Cataloguenumber
ENZ-769
Synonyms
kDagelatinase,GelatinaseA,Matrixmetalloproteinase-2,MMP-2,TBE-1,MMP2,CLG4A,CLG4,MONA,MMP-II.
Introduction
Matrixmetalloproteinase-2(MMP-2)isinvolvedinendometrialmenstrualbreakdown,regulationofvascularizationandtheinflammatoryresponse.MMP-2containsanumberofdistinctdomains:aprodomainthatiscleaveduponactivation;acatalyticdomaincontainingthezincbindingsite;afibronectinlikedomainbelievedtohavearoleinsubstratetargeting;andacarboxylterminal(hemopexinlike)domaincontaining2N-linkedglycosylation.TheMMP-2candegradeanextensivearrayofsubstratesincludingtypeIV,V,VIIandXcollagensaswellasgelatintypeI.Inaddition,MMP-2interactswithTHBS2,TIMP2,Thrombospondin1,CCL7andTIMP4.MMP-2autocatalyticcleavageintheC-terminalgeneratestheanti-angiogenicpeptide,PEX.ThisprocessseemstobemadepossIBLebybindingintegrinv/beta3.DefectsintheMMP-2arethecauseofTorg-Winchestersyndrome(TWS),akamulticentricosteolysisnodulosisandarthropathy(MONA).
Description
MMP2HumanRecombinantproducedinE.coliisasingle,non-glycosylatedpolypeptidechaincontaining576aminoacids(110-660a.a)andhavingamolecularmassof64.7kDa.MMP2isfusedtoa25aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
TheMMP2solution(0.25mg/ml)contains20mMTris-HClbuffer(pH8.0),0.15MNaCland10%glycerol.
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95%asdeterminedbySDS-PAGE.
References
Title:Thrombin-dependentMMP-2ActivityIsRegulatedbyHeparanSulfate
Publication:Journalof
BIOLOGicalChemistry
285.53(2010):41270-41279.Link:http://www.jbc.org/content/285/53/41270.full
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSEFYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGE
ADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLW
CSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADAWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.