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Prospec/MMP14 Human/Matrix Metalloproteinase-14 Recombinant Human/50µg/ENZ-1101-50µg-50µg
Cataloguenumber
ENZ-1101
Synonyms
MatrixMetallopeptidase14,MatrixMetallopeptidase14(Membrane-Inserted),Membrane-Type-1MatrixMetalloproteinase,MembraneType1Metalloprotease,EC3.4.24.80,MT-MMP1,MT1-MMP,MMP-14,MMP-X1,MT1MMP,MTMMP1,MatrixMetalloproteinase14(Membrane-Inserted),Membrane-TypeMatrixMetalloproteinase1,MatrixMetalloproteinase-14,EC3.4.24,MT-MMP,WNCHRS,Matrixmetalloproteinase-14,Membrane-typematrixmetalloproteinase1.
Introduction
Matrixmetalloproteinase-14(MMP14),isamembrane-anchoredzinc-bindingendopeptidasewhichisexpressedattheleADIngedgeofdifferentinvasivecarcinomasandalsopromotestumorcellinvasionthroughdegradationoftheextracellularmatrix.MMP14takesavitalpartinextracellularmatrix,ECM,remodelingbyhavingthecapABIlitytodegradetypeIcollagen,activatepro-MMP-2andprocesscelladhesionmoleculesforinstanceCD44andintegrinalphaV.MMP14isakeyenzymeinmanyphysiologicalaswellaspathologicalprocessesforexampleangiogenesis&tumorinvasion.
Description
MatrixMetalloproteinase-14HumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining264aminoacidsandhavingamolecularmassof29.6kDa.
MMP14ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
MMP14issuppliedasa0.2μmfilteredsolutionconteining20mMTris-HCl,pH7.4,30%glycerol,300mMNaCl,3mMCaCl2and10μMZnCl2.
Stability
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
ALASLGSAQSSSFSPEAWLQQYGYLPPGDLRTHTQRSPQSLSAAIAAMQKFYGLQVTGKADADTMKAMRRPRCGVPDKFGAEIKANVRRKRYAIQGLKWQHNEITFCIQNYTPKVGEYATYEAIRKAFRVWESATPLRFREVPYAYIREGHEKQADIMIFFAEGFHGDSTPFDGEGGFLAHAYFPGPNIGGDTHFDSAEPWTVRNEDLNGNDIFLVAVHELGHALGLEHSSDPSAIMAPFYQWMDTENFVLPDDDRRGIQQLYG.
SafetyDataSheet
SDS
