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Prospec/HPSE WB/Recombinant Human Heparanase-1 WB Control/200ng/ENZ-261-200ng-200ng
Cataloguenumber
ENZ-261
Introduction
Heparanaseisanendoβ-D-glucuronidase,whichdegradesheparansulfatesidechainsofheparansulfateproteoglycans(HSPGs)intheextracellularmatrix.HeparanaseplaysanimportantroleinECMdegradation,facilitatingthemigrationandextravasationoftumorcellsandinflammatoryleukocytes(1,2,3).Upondegradation,heparanasereleasesgrowthfactorsandcytokinesthatstimulatecellproliferationandchemotaxis(4,5).Heparanaseisaheterodimercomprisedofa50kDasubunitharboringtheactivesiteanda8kDasubunit.Itisproducedasalatent65kDaprecursorandproteolyticallyprocessedtoitsactiveform(1,6).Heparanaseishighlyexpressedinmyeloidleukocytes(i.e.neutrophils)inplateletsandinhumanplacenta.Humanheparanasewasfoundtobeupregulatedinvarioustypesofprimarytumors,correlatinginsomecaseswithincreasedtumorinvasivenessandvascularityandwithpoorProspectivesurvival(7,8).
Description
RecombinantHeparanaseproteinHPA1isproducedinCHOcells.Theproteinispurifiedbyseveralorthogonalchromatographysteps.
Formulation
Concentration:1μg/mlContent:100ngBuffer:LDS-PAGEbuffer[140mMTrisbufferpH8.5,10%Glycerol,2%LDS,0.015%EDTA,1.88%(v/v)of1%ServaBlueG250and0.625%(v/v)of1%Phenolred].
Applications
Positivecontrolforwesternblotanalysis.
Preparationprotocol
Use20μlofrecombinanthumanheparanase1(HPA1)perlane,asacontrolforusingmonoclonalantiHPA1cloneHP3/17antibodies(Cat.No.:Ins-AB-04001)orpolyclonalrabbitantiHPA1antibody(Cat.No.:Ins-AB-04002).
StorageProcedures
Storeat–20ºC,avoidrepeatedfreeze-thawcycles.
PatentProtectedCountries
RecombinantheparanaseisprotectedunderUS.PatentsNo.5,968,822;6,348,344;6,426,209,additionalUSpatentapplicationsandpatentsandpatentapplicationsworldwide.
References
1.I.Vlodavsky,Y.Friedmann,M.Elkin,H.Aingorn,R.Atzmon,R.Ishai-Michaeli,M.Bitan,O.Pappo,T.Peretz,I.Michal,L.Spector,I.Pecker.1999.Mammalianheparanase:genecloning,expressionandfunctionintumorprogressionandmetastasis.Nat.Med.5:793–802.2.I.Vlodavsky,Y.Friedman.2001.Molecularpropertiesandinvolvementofheparanaseincancermetastasisandangiogenesis.J.Clin.Invest.108:341–347.3.C.R.Parish,C.Freeman,M.D.Hulett.2001.Heparanase:akeyenzymeinvolvedincellinvasion.Biochem.Biophys.Acta1471:M99–M108.4.I.Vlodasvsky,G.Korner,R.Ishai-Michaeli,P.Bashkin,R.Bar-Shavit,Z.Fuks,1990.Extracellularmatrix-residentgrowthfactorsandenzyme:PossIBLeinvolvementintumormetastasisandangiogenesis.CancerMetastasisRev.9:203-226.5.P.Bashkin,S.Doctrow,M.Klagsbrun,C.M.Svahn,J.Folkman,I.Vlodavsky.1989.Basicfibroblastgrowthfactorbindstosubendothelialextracellularmatrixandisreleasedbyheparitinaseandheparin-likemolecules.Biochemistry28:1737-1743.6.M.B.Fairbanks,A.M.Mildner,J.W.Leone,G.S.Cavey,W.R.Mathews,R.F.Drong,J.L.Slightom,M.J.Bienkowski,C.W.Smith,C.A.Bannow,R.L.Heinrikson.1999.Processingofthehumanheparanaseprecursorandevidencethattheactiveenzymeisaheterodimer.J.Biol.Chem.274:29587–29590.7.A.Koliopanos,H.Friess,J.Klee.,X.Shi,Q.Liao,I.Pecker,I.Vlodavsky,A.Zimmermann,M.W.Buchler.1992.Heparanaseexpressioninprimaryandmetastaticpancreaticcancer.CancerRes.61:4655–4659.8.K.Gohji,H.Hirano,M.Okamoto,S.Kitazawa,M.Toyoshima,J.Dong,Y.Katsuoka,M.Nakajima.2001.Expressionofthreeextracellularmatrixdegradativeenzymesinbladdercancer.Int.J.Cancer95:295–301.
DataSheet
ToviewtheFULLVERSIONdatasheetclickHere:
SafetyDataSheet
SDS
Usage
ProSpec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
