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Prospec/GCK Human, Active/Hexokinase-4 Human Recombinant, Active/50µg/PKA-116-50µg-50µg
Cataloguenumber
PKA-116
Synonyms
Glucokinase,Glucokinase(Hexokinase4),HexokinaseTypeIV,HKIV,HK4,MaturityOnsetDiabetesofTheYoung2,ATP:D-Hexose6-Phosphotransferase,HexokinaseD,PancreaticIsozyme,Hexokinase-4,Hexokinase-D,Hexokinase4.
Introduction
GCKisanenzymethatexpeditetheformationofglucose-6-phosphateforglucosebyphosphorylation.HumansandothervertebrateshaveGCKinthecellsofthepancreasandliver.Inbothorgans,theenzymehasanimportantroleincarbohydratemetabolismregulationbysensingsugarlevelsandactingaccordingtothechangeinglucoselevels,thatcanriseafteramealorfallduringfasting.Mutationsinthegenethatcodesforthisenzymecancausehypoglycemiaordiabetes.
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Description
GCKHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining485aminoacids(1-465a.a)andhavingamolecularmassof54.3kDa.GCKisfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
GCKproteinsolution(1mg/ml)contains20mMTris-HClbuffer(pH8.0)and10%glycerol.
StABIlity
Purity
BIOLOGicalActivity
Specificactivityis>2,000pmol/min/ug.Oneunitwillconvert1pmolesofD-GlucosetoD-Glucose-6-phosphateperminuteatpH8.0at37C.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMLDDRARMEAAKKEKVEQILAEFQLQEEDLKKVMRRMQKEMDRGLRLETHEEASVKMLPTYVRSTPEGSEVGDFLSLDLGGTNFRVMLVKVGEGEEGQWSVKTKHQMYSIPEDAMTGTAEMLFDYISECISDFLDKHQMKHKKLPLGFTFSFPVRHEDIDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDHQCEVGMIVGTGCNACYMEEMQNVELVEGDEGRMCVNTEWGAFGDSGELDEFLLEYDRLVDESSANPGQQLYEKLIGGKYMGELVRLVLLRLVDENLLFHGEASEQLRTRGAFETRFVSQVESDTGDRKQIYNILSTLGLRPSTTDCDIVRRACESVSTRAAHMCSAGLAGVINRMRESRSEDVMRITVGVDGSVYKLHPSFKERFHASVRRLTPSCEITFIESEEGSGRGAALVSAVACKKACMLGQ.
SafetyDataSheet
SDS