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Prospec/MPG Human/N-Methylpurine-DNA Glycosylase Human Recombinant/2µg/ENZ-151-2µg-2µg
Cataloguenumber
ENZ-151
Synonyms
DNA-3-methyladenineglycosylase,3-alkyladenineDNAglycosylase,3-methyladenineDNAglycosidase,ADPG,N-methylpurine-DNAglycosylase,MPG,AAG,ANPG,MID1,MDG,PIG11,PIG16,CRA36.1.
Introduction
DNA-3-methyladenineglycosylase(MPG)isamemberoftheDNAglycosylaseMPGfamily.MPGinitiatesbaseexcisionrepairinDNAbyremovingawidevarietyofalkylated,deaminated,andlipidperoxidation-inducedpurineadducts.
Description
MPGHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining306aminoacids(1-298a.a.)andhavingamolecularmassof33.9kDa(MolecularweightonSDS-PAGEwillappearhigher).MPGisfusedtoan8aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
MPGproteinsolution(0.25mg/ml)containing20mMTris-HClbuffer(pH8.0),1mMDTT,30%glycerol,200mMNaCland1mMEDTA.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Aminoacidsequence
MVTPALQMKKPKQFCRRMGQKKQRPARAGQPHSSSDAAQAPAEQPHSSSDAAQAPCPRERCLGPPTTPGPYRSIYFSSPKGHLTRLGLEFFDQPAVPLARAFLGQVLVRRLPNGTELRGRIVETEAYLGPEDEAAHSRGGRQTPRNRGMFMKPGTLYVYIIYGMYFCMNISSQGDGACVLLRALEPLEGLETMRQLRSTLRKGTASRVLKDRELCSGPSKLCQALAINKSFDQRDLAQDEAVWLERGPLEPSEPAVVAAARVGVGHAGEWARKPLRFYVRGSPWVSVVDRVAEQDTQALEHHHHHH.
SafetyDataSheet
SDS