Cataloguenumber
PKA-344
Synonyms
Epidermalgrowthfactorreceptor,EC2.7.10.1,Receptortyrosine-proteinkinaseErbB-1,ERBB,mENA,ERBB1,EGFR.
Introduction
Theepidermalgrowthfactorreceptor(EGFR)subfamilyofreceptortyrosinekinasescomprisesfourmembers:EGFR(alsoknownasHER1,ErbB1orErbB),ErbB2(Neu,HER-2),ErbB3(HER-3),andErbB4(HER-4).AllfamilymembersaretypeItransmembraneglycoproteinthathasanextracellulardomainwhichcontainstwocysteine-richdomainsseparatedbyaspacerregionthatisinvolvedinligand-binding,andacytoplasmicdomainwhichhasamembrane-proximaltyrosinekinasedomainandaC-terminaltailwithmultipletyrosineautophosphorylationsites.ThehumanEGFRgeneencodesa1210aminoacid(aa)residueprecursorwitha24aaputativesignalpeptide,a621aaextracellulardomain,a23aatransmembranedomain,anda542aacytoplasmicdomain.EGFRhasbeenshowntobindasubsetoftheEGFfamilyligands,includingEGF,amphiregulin,TGF-a,betacellulin,epiregulin,HBEGFandneuregulin-2intheabsenceofaco-receptor.LigandbindinginducesEGFRhomodimerizationaswellasheterdimerizationwithErbB2,resultinginkinaseactivation,tyrosinephosphorylationandcellsignaling.EGFRcanalsoberecruitedtoformheterodimerswiththeligand-activatedErbB3orErbB4.EGFRsignalinghasbeenshowntoregulatemultipleBIOLOGicalfunctionsincludingcellproliferation,differentiation,motilityandapoptosis.Inaddition,EGFRsignalinghasalsobeenshowntoplayaroleincarcinogenesis.
Description
TheEGFRcontainstheextracellulardomainofthehumanEGFR(25-647a.a.)excludingthesignalpeptidewhichiscleavedbytheinsectcellshavinganapproximateMwof85kDa.TheEGFRisfusedtoaC-terminalStrep-tagandpurifiedbyproprietarychromatographictechniques.
Source
Sf9InsectCells.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
ErbB1waslyophilizedfromaconcentrated(1mg/ml)sterilesolutioncontaining1xPBSpH-7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedEGFRinsterilePBSnotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedEGFRalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionEGFRshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Aminoacidsequence
LEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDI
LKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCKLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIAASWSHPQFEK.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.