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Prospec/UGDH Mouse/UDP-Glucose Dehydrogenase Mouse Recombinant/1µg/ENZ-1070-1µg-1µg
Cataloguenumber
ENZ-1070
Synonyms
GDH,UDP-GlcDH,UDPGDH,UGD,EC=1.1.1.22,UDP-Glcdehydrogenase,UDP-glucose6-dehydrogenase,UGDH.
Introduction
UGDHispartoftheUDP-glucose/GDP-mannosedehydrogenasefamilyandisawidelyexpressedenzymelocalizedintheliver.UGDHtransfersUDP-glucosetoUDP-glucuronateandthustakespartinthebiosynthesisofglycosaminoglycanssuchashyaluronan,chondroitinsulfate,andheparansulfate.Theseglycosylatedproductsareordinarymoleculesoftheextracellularmatrixandparticipateinsignaltransduction,cellmigration,andcancergrowthandmetastasis.
Description
UGDHMouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining516aminoacids(1-493a.a.)andhavingamolecularmassof57.2kDa.UGDHisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
UGDHproteinsolution(0.5mg/ml)containing20mMMESbuffer(pH5.0),20%glycerol150mMNaCland1mMEDTA.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMVEIKKICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNEARINAWNSPTLPIYEPGLKEVVESCRGKNLFFSTNIDDAIREADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDRVLIGGDETPEGQKAVRALCAVYEHWVPKEKILTTNTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVIDMNDYQRRRFASRIIDSLFNTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDPKVPREQIVVDLSHPGVSADDQVSRLVTISKDPYEACDGAHALVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDGLHSELQTIGFQIETIGKKVSSKRIPYTPGEIPKFSLQDPPNKKPKV.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
Specificactivityis>2,500pmol/min/ug,andisdefinedastheamountofenzymethatconverts1.0pmoleofUDP-glucosetoUDP-glucuronateperminuteatpH8.7at37°C.
SafetyDataSheet
SDS
