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当前位置: 首页 > 产品中心 > Other_enzymes > Prospec/MDH1 Chicken/Malate Dehydrogenase Chicken Recombinant/50000IU/ENZ-273-50000IU-50000IU
商品详细Prospec/MDH1 Chicken/Malate Dehydrogenase Chicken Recombinant/50000IU/ENZ-273-50000IU-50000IU
Prospec/MDH1 Chicken/Malate Dehydrogenase Chicken Recombinant/50000IU/ENZ-273-50000IU-50000IU
Prospec/MDH1 Chicken/Malate Dehydrogenase Chicken Recombinant/50000IU/ENZ-273-50000IU-50000IU
商品编号: ENZ-273-50000IU-50000IU
品牌: prospecbio
市场价: ¥19800.00
美元价: 8415.00
产地: 美国(厂家直采)
公司:
产品分类: 其它酶
公司分类: Other_enzymes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Catalogue number

ENZ-273

Synonyms

Malate dehydrogenase cytoplasmic, EC 1.1.1.37, Cytosolic malate dehydrogenase, MDHA, MOR2, MDH-s, MGC:1375, MDH1.

Introduction

Malate dehydrogenase (EC1.1.1.37) is an enzyme in the citric acid cycle that catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is not to be confused with malic enzyme, which catalyzes the conversion of pyruvate using NADPH.Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by pyruvate carboxylase to form oxaloacetate, a citric acid cycle intermediate. In order to get the oxaloacetate out of the mitochondria, malate dehydrogenase reduces it to malate, and it then traverses the inner mitochondrial membrane. Once in the cytosol, the malate is oxidized back to oxaloacetate by cytosolic malate dehydrogenase. Finally, phosphoenol-pyruvate carboxy kinase (PEPCK) converts oxaloacetate to phosphoenol pyruvate.

Description

The DNA encoding Malate (Malic) Dehydrogenase is cloned from cDNA library of chicken heart.The MDH1 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile lyophilized powder.

Formulation

Each mg of protein contains 0.59mg NaPO4.

Solubility

It is recommended to reconstitute the lyophilized Malate dehydrogenase in sterile 18MΩ-cm H2O.

Stability

Lyophilized Malate dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MDH1 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Unit Definition

One unit is defined as 1 umol of NAD+ production per minute under the assay conditions (25°C, pH 7.5).

Specific Activity

Specific Activity Greater than 710U/mg protein.

Safety Data Sheet

SDS

Usage

Prospec"s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
品牌介绍
    ProSpec-Tany TechnoGene Ltd.公司创立人Dr. Tanchum (Tany) Amarant 主要致力于蛋白生物化学方面的研究工作,1947年出生于以色列。1991-1997创建ReProGen 公司,从事细胞因子和趋化因子的大量生产及纯化;1997-2000创建ProSpec-TechnoGene,主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利United States Patent 5434135。       ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research.       Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more.....       Our research team and scientists have been able to assemble more than 1700 recombinant proteins, peptides and antibodies for your research needs. The proteins are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices.       ProSpec has established sterling reputation among the scientific community, a reputation built on innovative products, customer service and attention to detail. Our reputation for quality is an essential factor in achieving a favorable position in the market place and the benefits associated with manufacturing top quality products.