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Prospec/LDHB Mouse/Lactate Dehydrogenase B Mouse Recombinant/5µg/ENZ-1052-5µg-5µg
Cataloguenumber
ENZ-1052
Synonyms
L-lactatedehydrogenaseBchain,LDH-B,LDHheartsubunit,LDH-H,Ldh-2,Ldh2,Ldhb.
Introduction
Lactatedehydrogenase(LDH)isanenzyme(EC1.1.1.27)presentinawidevarietyoforganisms,includingplantsandanimals.AtetramericenzymethatcatalysestheinterconversionofpyruvateandlactatewithconcomitantinterconversionofNADHandNAD+.Athighconcentrationsofpyruvate,theenzymeexhibitsfeedbackinhibitionandtherateofconversionofpyruvatetolactateisdecreased.Invertebrates,genesforthreedifferentsubunits(LDH-A,LDH-BandLDH-C)exist.
Description
LDHBMouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining357aminoacids(1-334a.a)andhavingamolecularmassof39kDa.LDHBisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
LDHBproteinsolution(1mg/ml)containing20mMTris-HCl(pH8.0),1mMDTTand10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorage,itisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
Specificactivityis>150units/mg,inwhichoneunitwill1.0umoleofpyruvatetoL-lactateandbeta-NADperminuteatpH7.5.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMATLKEKLIASVADDEAAVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCTIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVDSAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLRKSADTLWDIQKDLKDL.
SafetyDataSheet
SDS