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Prospec/DDT Mouse/D-Dopachrome Tautomerase Mouse Recombinant/20µg/ENZ-1073-20µg-20µg
Cataloguenumber
ENZ-1073
Synonyms
D-dopachromedecarboxylase(EC:4.1.1.84),D-dopachrometautomerase,Ddt.
Introduction
DDTisanenzymethatcatayzesthetautomerizationofD-dopachrometogive5,6-dihydroxyindole(DHI).DDTispartofthefamilyoflyases,specificallythecarboxy-lyases,whichcleavecarbon-carbonbonds.DDTsharesahomologousaminoacidsequence(33%identical)withMIFandhassimilartautomeraseactivity.DDTfunctionsaproinflammatorycytokine.
Description
DDTMouseRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining141aminoacids(1-118a.a)andhavingamolecularmassof15.5kDa.DDTisfusedtoa23aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
DDTproteinsolution(1mg/ml)contains20mMTris-Hclbuffer(pH8.0)&10%glycerol.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMGSMPFVELETNLPASRIPAGLENRLCAATATILDKPEDRVSVTIRPGMTLLMNKSTEPCAHLLVSSIGVVGTAEQNRTHSASFFKFLTEELSLDQDRIVIRFFPLEAWQIGKKGTVMTFL
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.