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当前位置: 首页 > 产品中心 > Other_enzymes > Prospec/LACTB E.coli/Beta Lactamase E.coli Recombinant/10mg/ENZ-351-10mg-10mg
商品详细Prospec/LACTB E.coli/Beta Lactamase E.coli Recombinant/10mg/ENZ-351-10mg-10mg
Prospec/LACTB E.coli/Beta Lactamase E.coli Recombinant/10mg/ENZ-351-10mg-10mg
Prospec/LACTB E.coli/Beta Lactamase E.coli Recombinant/10mg/ENZ-351-10mg-10mg
商品编号: ENZ-351-10mg-10mg
品牌: prospecbio
市场价: ¥18000.00
美元价: 7650.00
产地: 美国(厂家直采)
公司:
产品分类: 其它酶
公司分类: Other_enzymes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Catalogue number

ENZ-351

Synonyms

b-Lactamase, EC 3.5.2.6, TEM-1.

Introduction

Beta-lactamase is a type of enzyme (EC 3.5.2.6) produced by some bacteria that is responsible for their resistance to beta-lactam antibiotics like penicillins, cephalosporins, cephamycins and carbapenems. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule"s antibacterial properties.

Description

Recombinant E.coli Beta-Lactamase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids and having a molecular mass of 29 kDa. Beta Lactamase is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM Phosphate buffer pH-7.

Solubility

It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18MΩ-cm H2O at a concentration of 100 µg/ml, which can then be further diluted to other aqueous solutions. The Beta Lactamase should be used in pH 7.0- 8.0 and in temperature not higher then 45°c.

Stability

Lyophilized Beta Lactamase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Beta Lactamase Recombinant should be storedat 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Purity

Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Amino acid sequence

MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVRELCSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW.

Specific Activity

700IU/mg.

Unit Definition

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25°C.The International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Safety Data Sheet

SDS

Usage

ProSpec"s products are furnished forLABORATORY RESEARCHUSEONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.

品牌介绍
    ProSpec-Tany TechnoGene Ltd.公司创立人Dr. Tanchum (Tany) Amarant 主要致力于蛋白生物化学方面的研究工作,1947年出生于以色列。1991-1997创建ReProGen 公司,从事细胞因子和趋化因子的大量生产及纯化;1997-2000创建ProSpec-TechnoGene,主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利United States Patent 5434135。       ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research.       Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more.....       Our research team and scientists have been able to assemble more than 1700 recombinant proteins, peptides and antibodies for your research needs. The proteins are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices.       ProSpec has established sterling reputation among the scientific community, a reputation built on innovative products, customer service and attention to detail. Our reputation for quality is an essential factor in achieving a favorable position in the market place and the benefits associated with manufacturing top quality products.