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Prospec/AHCY Human, Sf9 /Adenosylhomocysteinase Human Recombinant, Sf9/5µg/ENZ-1034-5µg-5µg
Cataloguenumber
ENZ-1034
Synonyms
EC3.3.1.1,SAHH,AdoHcyase,S-adenosyl-L-homocysteinehydrolase,AHCY,Adenosylhomocysteinase.
Introduction
AHCYisanenzymethatcatalyzesthereversIBLehydrolysisofS-adenosylhomocysteine(AdoHcy)toadenosine(Ado)andL-homocysteine(Hcy).AHCYcontrolstheintracellularS-adenosylhomocysteine(SAH)concentrationthatiscrucialfortransmethylationreactions.AHCYdeficiencycauseshypermethioninemia.
Description
AHCYHumanRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining441aminoacids(1-432a.a.)andhavingamolecularmassof48.8kDa(Migratesat40-57kDaonSDS-PAGEunderreducingconditions).AHCYisfusedtoa6aminoacidsHis-TagatC-terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
AHCYproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADLMSDKLPYKVADIGLAAWGRKALDIAENEMPGLMRMRERYSASKPLKGARIAGCLHMTVETAVLIETLVTLGAEVQWSSCNIFSTQDHAAAAIAKAGIPVYAWKGETDEEYLWCIEQTLYFKDGPLNMILDDGGDLTNLIHTKYPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCIDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRLKNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHYRYHHHHHH.
SafetyDataSheet
SDS