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Prospec/YWHAE Human, His/Tyr-3/Trp-5 Monooxygenase Activation Protein, Epsilon Human Recombinant, His Tag/100µg/PKA-095-100µg-100µg
Cataloguenumber
PKA-095
Synonyms
YWHAE,MDS,MDCR,KCIP-1,14-3-3E,14-3-3Epsilon,FLJ45465,Tyr-3/Trp-5MonooxygenaseActivationProteinEpsilon,HEL2,KCIP-1,MDCR,MDS.
Introduction
The14-3-3familyofproteinsplaysakeyregulatoryroleinsignaltransduction,checkpointcontrol,apoptoticandnutrient-sensingpathways.14-3-3proteinsarehighlyconservedandubiquitouslyexpressed.Thereareatleastsevenisoforms,β,γ,ε,σ,ζ,τandηthathavebeenidentifiedinmammals.The14-3-3epsilon,asubtypeofthe14-3-3familyofproteins,wasthoughttobebrainandneuron-specific.IthasbeenshowntointeractwithCDC25phosphatases,RAF1andIRS1proteins,suggestingitsroleindiversebiochemicalactivitiesrelatedtosignaltransduction,suchascelldivisionandregulationofinsulinsensitivity.Ithasalsobeenimplicatedinthepathogenesisofsmallcelllungcancer.
Description
YWHAEHumanRecombinantproducedinE.coliisasinglepolypeptidechaincontaining275aminoacids(1-255)andhavingamolecularmassof31.3kDa.YWHAEisfusedtoa20aminoacidHis-tagatN-terminus&purifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
TheYWHAEsolution(1mg/1ml)containsphosphatebufferedSaline(pH7.4),1mMDTTand10%glycerol.
StABIlity
Purity
Aminoacidsequence
MGSSHHHHHHSSGLVPRGSHMDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ.
SafetyDataSheet
SDS