Cataloguenumber
HOR-269
Introduction
Exedin-4Recombinantisalikepeptide-1receptoragoNIST.ThenativehormoneisproducedinthegutofGilamonsterHelodermasUSPectrum(atypeofreptilefoundinthedesert)thatstimulatesproductionwithoutcausingthreateninglylowbloodsugar,whichcanoccurafterusingsomeanti-diabetesproducts.Recently,researchersusedextractedsalivafromgilamonsterstocreateanunprecedentedbreakthroughinDiabetesType2treatment.Unlikeotherproductstakenfortype2diabetes,Exedin-4hasnotbeenlinkedwithweightgainandactuallyresultedinweightloss,accordingtotheresearchers.Exedin-4enhancesglucose-dependantsecretion,suppressesinappropriatelyelevatedsecretionandslowsgastricemptyinginvivo.ItalsopromotesB-cellproliferationandneogenesisinvitroandinanimalmodels.Exedin-4stimulatesanincreaseinacinarcAMP,withoutstimulatingthereleaseofamylase.
Description
Exendin-4Recombinantisanovel39-aminoacidspeptidehavingamolecularmassof4186.7Dalton,whichshares53%,sequencehomologywithGLP-17-36amideandinteractswiththesamemembranereceptor.TheExendin-4ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
TheExendin-4peptidewaslyophilizedafterextensivedialysesagainst20mMPBSandcontains4%mannitol.
Solubility
ItisrecommendedtoreconstitutethelyophilizedExendinRecombinantinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedExendinRecombinantalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionExendin-4shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
ThesequencedeterminedandwasfoundtobeHis-Gly-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Leu-Ser-Lys-Gln-Met-Glu-Glu-Glu-Ala-Val-Arg-Leu-Phe-Ile-Glu-Trp-Leu-Lys-Asn-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser.
1.RegulatesGlucoselevelsrapidly2.ReducesGLCN.3.ReducesHbA1c4.Stimulatesbetacellgrowthproduction.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.