Catalogue number
CYT-257
Synonyms
Neurotrophic factor, Nerve growth factor-2, NGF-2, HDNF, NT-3.
Introduction
NT3 a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
Description
Neurotrophin-3 Human Recombinant produced in E.Coli is a non-glycosylated and non-covalently linked homodimer, containing 2x119 amino acid chains, having a total Mw of 27.2 kDa.The NT-3 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from 0.02% TFA.
Solubility
It is recommended to reconstitute the lyophilized Neurotrophin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized NGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity
Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino acid sequence
YAEHKSHRGE YSVCDSESLW VTDKSSAIDI RGHQVTVLGE IKTGNSPVKQ YFYETRCKEA RPVKNGCRGI DDKHWNSQCK TSQTYVRALT SENNKLVGWR WIRIDTSCVC ALSRKIGRT.
Biological Activity
The ED50 as determined by the dose-dependent proliferation of C6 cells and is 3.6-5.4?g/ml.
Protein content
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 2.165 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of NT-3 as a Reference Standard.
Usage
Prospec"s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Safety Data Sheet
References
1.Title:Generation of Neurons with Improved Cell Survival and Phenotype Maintenance Using a Degradation-Resistant Nurr1 Mutant[1]^†[2]^‡.Publication:Article first published online: 11 JUN 2009 DOI: 10.1002/stem.146 Copyright © 2009 AlphaMed Press.Link:http://onlinelibrary.wiley.com/doi/10.1002/stem.146/fullLinks:1.http://onlinelibrary.wiley.com/doi/10.1002/stem.146/full#fn12.http://onlinelibrary.wiley.com/doi/10.1002/stem.146/full#fn3
2.Title:Mash1 and Neurogenin 2 Enhance Survival and Differentiation of Neural Precursor Cells After Transplantation to Rat Brains via Distinct Modes of Action.Publication: Received 22 May 2008; Accepted 8 August 2008; Published online 9 September 2008.Molecular Therapy (2008) 16 11, 1873–1882 doi:10.1038/mt.2008.189Link:http://www.nature.com/mt/journal/v16/n11/full/mt2008189a.html
3.Title: TrkA Receptor “Hot Spots” for Binding of NT-3 as a Heterologous Ligand Publications: Journal of Biological Chemistry 282.23 (2007): 16754-16763. Link: http://www.jbc.org/content/282/23/16754.full
4.Title: Orphan nuclear receptor Nurr1 induces neuron differentiation from embryonic cortical precursor cells via an extrinsic paracrine mechanismPublication: FEBS letters 583.9 (2009): 1505-1510Linkhttp://www.sciencedirect.com/science/article/pii/S0014579309002828
