Cataloguenumber
PRO-2428
Synonyms
CD320antigenisoform1,CD320,8D6,8D6A,TCBLR,FDC-SM-8D6,FDC-signalingmolecule8D6.
Introduction
CD320isapartofthelow-densitylipoproteinreceptorfamily,having2low-densitylipoproteinreceptortypeAdomainsdividedbyacomplement-likecysteine-richregion.CD320participatesincobalaminuptakeandinternalizesthecomplexbyendocytosis.CD320isalsoplaysaroleascostimulatortopromoteBcellandfolliculardendriticcellsresponses.CD320isalsoknowntoaugmenttheproliferationofPCprecursorsgeneratedbyIL-10.
Description
CD320producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining438aminoacids(31-229a.a.)andhavingamolecularmassof47.4kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately57-70kDa).CD320isexpressedwitha239aminoacidhIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
CD320proteinsolution(0.5mg/ml)containingPhosphateBufferedSaline(pH7.4),and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
LEAAASPLSTPTSAQAAGPSSGSCPPTKFQCRTSGLCVPLTWRCDRDLDCSDGSDEEECRIEPCTQKGQCPPPPGLPCPCTGVSDCSGGTDKKLRNCSRLACLAGELRCTLSDDCIPLTWRCDGHPDCPDSSDELGCGTNEILPEGDATTMGPPVTLESVTSLRNATTMGPPVTLESVPSVGNATSSSAGDQSGSPTAYLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
SafetyDataSheet
SDS