Cataloguenumber
PRO-2429
Synonyms
Basalcelladhesionmoleculeisoform1,BCAM,AU,CD239,LU,MSK19,AubergerBantigen,B-CAMcellsurfaceglycoprotein,F8/G253antigen,Lutheranantigen,Lutheranbloodgroupglycoprotein,CD_antigen:CD239,LU,MSK19.
Introduction
BasalCellAdhesionMolecule(BCAM)whichisaproductofalternatesplicingoftheLutheranbloodgroupmoleculeisapartoftheimmunoglobulinsuperfamily.BCAMcontainsfiveextracellularimmunoglobulindomains,asingletransmembranedomain,andashortC-terminalcytoplasmictail.BCAMproteinisupregulatedfollowingmalignanttransformationofsomecelltypesinvivoandinvitro.FurThermore,BCAMinteractswithintegrininsickleredcells,andparticipatesinvasoocclusiveepisodes.
Description
BCAMproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining755aminoacids(32-547a.a.)andhavingamolecularmassof83.2kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately70-100kDa).BCAMisexpressedwitha239aminoacidhIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Formulation
BCAMproteinsolution(0.5mg/ml)containingPhosphateBufferedSaline(pH7.4),and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
EVRLSVPPLVEVMRGKSVILDCTPTGTHDHYMLEWFLTDRSGARPRLASAEMQGSELQVTMHDTRGRSPPYQLDSQGRLVLAEAQVGDERDYVCVVRAGAAGTAEATARLNVFAKPEATEVSPNKGTLSVMEDSAQEIATCNSRNGNPAPKITWYRNGQRLEVPVEMNPEGYMTSRTVREASGLLSLTSTLYLRLRKDDRDASFHCAAHYSLPEGRHGRLDSPTFHLTLHYPTEHVQFWVGSPSTPAGWVREGDTVQLLCRGDGSPSPEYTLFRLQDEQEEVLNVNLEGNLTLEGVTRGQSGTYGCRVEDYDAADDVQLSKTLELRVAYLDPLELSEGKVLSLPLNSSAVVNCSVHGLPTPALRWTKDSTPLGDGPMLSLSSITFDSNGTYVCEASLPTVPVLSRTQNFTLLVQGSPELKTAEIEPKADGSWREGDEVTLICSARGHPDPKLSWSQLGGSPAEPIPGRQGWVSSSLTLKVTSALSRDGISCEASNPHGNKRHVFHFGTVSPQTSQAVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
SafetyDataSheet
SDS