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Prospec/CD14 Human, CHO/CD14 Human Recombinant, CHO/50µg/PRO-536-50µg-50µg
Prospec/CD14 Human, CHO/CD14 Human Recombinant, CHO/50µg/PRO-536-50µg-50µg
商品编号：
PRO-536-50µg-50µg
品牌：
prospecbio
市场价：
¥2600.00
美元价：
1170.00
产品分类：
人体抗原
公司分类：
human_antigen
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Catalogue number
PRO-536
Synonyms
Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.
Introduction
CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.
Description
The CD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50-kDa protein containing 335 amino acids and lacks the GPI-Anchor.
Source
CHO-cells.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
CD14 was lyophilized from a concentrated protein solution (1.0 mg/ml) containing phosphate-buffered saline, pH 7.2.
Solubility
It is recommended to reconstitute the lyophilized CD14 in sterile 18MΩ-cm H2O not less than 100µg/ml. Further dilutions should be made with phosphate buffered saline (PBS).
Stability
Lyophilized CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD14 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
Up to 20 µg/ml CD14 inhibit binding of FITC-LPS (0.5µg/ml) to 600,000 CD14+CHO transfectants (FACS).
Safety Data Sheet
SDS
Usage
Prospec"s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
品牌介绍
ProSpec-Tany TechnoGene Ltd.公司创立人Dr. Tanchum (Tany) Amarant 主要致力于蛋白生物化学方面的研究工作，1947年出生于以色列。1991-1997创建ReProGen 公司，从事细胞因子和趋化因子的大量生产及纯化；1997-2000创建ProSpec-TechnoGene，主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利United States Patent 5434135。
ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research.
Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more.....
Our research team and scientists have been able to assemble more than 1700 recombinant proteins, peptides and antibodies for your research needs. The proteins are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices.
ProSpec has established sterling reputation among the scientific community, a reputation built on innovative products, customer service and attention to detail. Our reputation for quality is an essential factor in achieving a favorable position in the market place and the benefits associated with manufacturing top quality products.
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