Catalogue number
CHM-350
Synonyms
Introduction
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets. Its major physiologic role appears to be neutralization of molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair. Oncostatin-A is a member of the CXC chemokine family. Furthermore it is used as an inhibitor in the angiogenesis during tumor therapy.
Description
Source
Physical Appearance
Formulation
The CXCL4 protein was lyophilized after extensive dialysis against 50mM Tris-HCl pH 8.0 and 150mM NaCl buffer.
Solubility
It is recommended to reconstitute the lyophilized CXCL4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Purity
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino acid sequence
The sequence of the first four N-terminal amino acids was determined and was found to be Glu-Ala-Glu-Glu-Asp.
Biological Activity
The ED50 of CXCL4 as determined by its ability to inhibit human FGF basic dependent proliferation of NR6R3T3 mouse fibroblasts was found to be 5-15 μg/ml.
Safety Data Sheet
SDS
Usage
ProSpec"s products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs,agricultural or pesticidal products, food additives or household chemicals.
