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Prospec/PF 4 Human/Platelet Factor-4 Human Recombinant (CXCL4)/5µg/CHM-350-5µg-5µg
Cataloguenumber
CHM-350
Synonyms
Introduction
Plateletfactor-4isa70-aminoacidproteinthatisreleasedfromthealpha-granulesofactivatedplatelets.Itsmajorphysiologicroleappearstobeneutralizationofmoleculesontheendothelialsurfaceofbloodvessels,therebyinhibitinglocalantithrombinIIIactivityandpromotingcoagulation.Asastrongchemoattractantforneutrophilsandfibroblasts,PF4probablyhasaroleininflammationandwoundrepair.Oncostatin-AisamemberoftheCXCchemokinefamily.FurThermoreitisusedasaninhibitorintheangiogenesisduringtumortherapy.
Description
Source
PhysicalAppearance
Formulation
TheCXCL4proteinwaslyophilizedafterextensivedialysisagainst50mMTris-HClpH8.0and150mMNaClbuffer.
Solubility
ItisrecommendedtoreconstitutethelyophilizedCXCL4insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
ThesequenceofthefirstfourN-terminalaminoacidswasdeterminedandwasfoundtobeGlu-Ala-Glu-Glu-Asp.
BIOLOGicalActivity
TheED50ofCXCL4asdeterminedbyitsabilitytoinhibithumanFGFbasicdependentproliferationofNR6R3T3mousefibroblastswasfoundtobe5-15μg/ml.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.