Cataloguenumber
CYT-1063
Synonyms
Introduction
Prolactinisaneuroendocrinehormonesynthesizedprimarilybythepituitaryglandbutalsoavarietyofothercelltypesincludingtheplacenta,brainanduterus.Prolactinissecretedwheneating,nursing,mating,estrogentreatmentandduringovulation.Prolactin"sprimaryroleistopromoteandmaintainlactationbutalsoplaysaroleinbreastcancerdevelopment,regulationofreproductivefunctionandimmunoregulation.
Description
ProlactinHumanRecombinantPegylatedproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining199aminoacids+anadditionalAlaatn-terminal.PegylatedProlactinismono-pegylatedhavingamolecularmassof~39kDa,howeverundernon-denaturingconditionsitbehavesas220kDaproteinduetoitsincreasedhydrodynamicvolume.
ThePegylatedProlactinproteinispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutionwith0.02%NaHCO3.
Solubility
ItisrecommendedtoreconstitutethelyophilizedProlactininsterile0.4%NaHCO3pH-8.5,notlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedProlactinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionProlactinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbygelfiltrationanalysis.
BIOLOGicalActivity
PegylatedHumanProlactinwastestedforitsbiologicalfunctionalityin-vitrobyinducingproliferationofNb2cellsorBaf/3cellsthatwerestablytransfectedwithHumanProlactinreceptors,thoughitsactivityislowerthanhumanProlactin.However,itisanticipatedthatitsbiologicalactivityinvivowillbehigherthanhumanProlactinduetoprolongedpersistenceincirculation.
SafetyDataSheet
SDS