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Prospec/Prolactin Human, Antagonist/Prolactin Antagonist Human Recombinant/5µg/CYT-1066-5µg-5µg
Cataloguenumber
CYT-1066
Synonyms
Introduction
Prolactinisaneuroendocrinehormonesynthesizedprimarilybythepituitaryglandbutalsoavarietyofothercelltypesincludingtheplacenta,brainanduterus.Prolactinissecretedwheneating,nursing,mating,estrogentreatmentandduringovulation.Prolactin"sprimaryroleistopromoteandmaintainlactationbutalsoplaysaroleinbreastcancerdevelopment,regulationofreproductivefunctionandimmunoregulation.
Description
ProlactinHumanRecombinantAntagoNISTdes1-9,G129RproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining190aminoacids+anadditionalAlaatn-terminalandhavingamolecularmassof~22kDa.
TheHumanProlactinAntagonistispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutionwith0.02%NaHCO3
Solubility
ItisrecommendedtoreconstitutethelyophilizedProlactininsterile0.4%NaHCO3pH-8.5,notlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedProlactinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionProlactinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbygelfiltrationanalysis.
Aminoacidsequence
Thea.a.sequenceofthe1st6N-terminala.a.wasfoundtobeAla-Arg-Ser-Gln-Val-Thr.
BIOLOGicalActivity
HumanProlactinAntagonistwastestedforitsbiologicalfunctionalityin-vitrobyinducingproliferationofNb2cellsorBaf/3cellsthatwerestablytransfectedwithHumanProlactinreceptors.ProlactinAntagonistalsointeractsat1:1molarratiowithhumanprolactinreceptorextracellulardomainasdocumentedbySECandSPR(Biacoreanalysis).
SafetyDataSheet
SDS
