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Prospec/PLGF2 Human/Placental Growth Factor-2 Human Recombinant/10µg/CYT-1116-10µg-10µg
Cataloguenumber
CYT-1116
Synonyms
PIGF,PGF,PlGF-2,PLGF-2.
Introduction
PLGFisagrowthfactoractiveinangiogenesis,andendothelialcellgrowth,stimulatingtheirproliferationandmigration.Itbindstoreceptorvegfr-1/flt1.PLGF-2bindsneuropilin-1and2inadependentway.
Description
PlacentalGrowthFactor-2HumanRecombinantproducedinE.Coliisanon-glycosylated,disulfide-linkedhomodimerconsistingof2x152aminoacidpolypeptidechains,havingatotalmolecularmassofapproximately34.6kDa.
PLGF2ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutioninPBS,pH7.4and0.02%Tween-20.
Solubility
ItisrecommendedtoreconstitutethelyophilizedPlacentalGrowthFactor-2insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedPLGF2althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionPlacentalGrowthFactor-2shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
LPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALERLVDVVSEYPSEVEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQLLKIRSGDRPSYVELTFSQHVRCECRPLREKMKPERRRPKGRGKRRREKQRPTDCHLCGDAVPRR.
BIOLOGicalActivity
Determinedbyitsabilitytochemoattracthumanmonocytesusingaconcentrationrangeof5.0-50ng/ml.
SafetyDataSheet
SDS