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Prospec/PLGF1 Human, 132 a.a./Placental Growth Factor-1, 132 a.a. Human Recombinant/2µg/CYT-1133-2µg-2µg
Cataloguenumber
CYT-1133
Synonyms
PIGF,PGF,PLGF-1.
Introduction
PLGFisagrowthfactoractiveinangiogenesis,andendothelialcellgrowth,stimulatingtheirproliferationandmigration.Itbindstoreceptorvegfr-1/flt1.
Description
PlacentalGrowthFactor-1HumanRecombinantproducedinE.Coliisanon-glycosylated,disulfide-linkedhomodimerconsistingof2x132aminoacidpolypeptidechains,havingatotalmolecularmassofapproximately29.7kDa.
PLGF1ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2μmfilteredconcentratedsolutionin10mMSodiumPhosphatepH7.5.
Solubility
ItisrecommendedtoreconstitutethelyophilizedPlacentalGrowthFactor-1insterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedPLGF1althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionPlacentalGrowthFactor-1shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
MLPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALERLVDVVSEYPSEVEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQLLKIRSGDRPSYVELTFSQHVRCECRPLREKMKPERCGDAVPRR.
BIOLOGicalActivity
Determinedbyitsabilitytochemoattracthumanmonocytesofusingaconcentrationrangeof1.0-10.0ng/ml.
SafetyDataSheet
SDS