Cataloguenumber
CYT-501
Synonyms
Glioma-derivedgrowthfactor,GDGF,Osteosarcoma-derivedGrowthFactor,ODGF,SIS,SSV,PDGF2,c-sis,FLJ12858,PDGF-BB,PDGFB-chain,Platelet-derivedgrowthfactorbetapolypeptide.
Introduction
PDGF-BBisamemberoftheplatelet-derivedgrowthfactorfamily.Thefourmembersofthisfamilyaremitogenicfactorsforcellsofmesenchymaloriginandarecharacterizedbyamotifofeightcysteines.Thisgeneproductcanexisteitherasahomodimer(PDGF-BB)orasaheterodimerwiththeplatelet-derivedgrowthfactoralphapolypeptide(PDGF-AB),wherethedimersareconnectedbydisulfidebonds.Mutationsinthisgeneareassociatedwithmeningioma.Reciprocaltranslocationsbetweenchromosomes22and7,atsiteswherethisgeneandthatforCOL1A1arelocated,areassociatedwithaparticulartypeofskintumorcalleddermatofibrosarcomaprotuberansresultingfromunregulatedexpressionofgrowthfactor.Twosplicevariantshavebeenidentifiedforthisgene.
Description
Platelet-DerivedGrowthFactorBBHumanRecombinantisahomodimeric,non-glycosylated,polypeptidechaincontaining2x109aminoacids(218aminoacidsintotal)andhavingamolecularmassof24.3kDa.PDGF-BBispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedfroma0.2µmfilteredconcentratedsolutioninPBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedPlatelet-derivedGrowthFactor-BBinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedPlatelet-derivedGrowthFactorBBalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionPDGFBBshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT.
Determinedbythedose-dependentstimulationoftheproliferationofBalb/c3T3cells.TheexpectedED50forthiseffectis1.0-3.0ng/ml.
References
1.Title:Platelet-derivedgrowthfactormediatessurvivalofleukemiclargegranularlymphocytesviaanautocrineregulatorypathway.Publication:PublishedonlinebeforeprintOctober30,2009,doi:10.1182/blood-2009-06-223719BloodJanuary7,2010vol.115no.151-60.Link:http://bloodjournal.hematologylibrary.org/content/115/1/51.full2.Title:attenuatesskeletalmuscledystrophyinmdxmice.Publication:PublishedonlinebeforeprintMarch16,2009,doi:10.1096/fj.09-129833August2009TheFASEBJournalvol.23no.82539-2548.Link:http://www.fasebj.org/content/23/8/2539.full
3.Title:HumanCordBlood-DerivedEndothelialProgenitorCellsandTheirConditionedMediaExhibitTherapeuticEquivalenceforDiabeticWoundHealing.Publication:CellTransplantation,Vol.19,pp.1635–1644,20100963-6897/10$90.00+.00
PrintedintheUSA.Allrightsreserved.DOI:10.3727/096368910X516637
Copyright?2010CognizantComm.Corp.
Link:http://docserver.ingentaconnect.com/deliver/connect/cog/09636897/v19n12/s11.pdf?expires=1370351771&id=74450861&titleid=5476&accname=Guest+User&checksum=14BACCDE2ACF33
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.