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Prospec/NOV Human, HEK/Nephroblastoma Overexpressed Human Recombinant, HEK/2µg/CYT-1032-2µg-2µg
Cataloguenumber
CYT-1032
Synonyms
ProteinNOVhomolog,NovH,CCNfamilymember3,nsulin-likegrowthfactor-bindingprotein9,IBP-9,IGF-bindingprotein9,IGFBP-9,Nephroblastoma-overexpressedgeneproteinhomolog,NOV,CCN3,IGFBP9,NOVH.
Introduction
NephroblastomaOverexpressed(NOV)whichisencodedbytheNOVgeneisapartoftheCCN(CTGF/CYR61/NOV)family.NOVtakespartinreducingtumorgenicityandproliferationofcertaincancercelllines.NOVinteractswithnumerousproteinsandisinvolvedinbothinternalandexternalcellsignaling.NOVisexpressedinparticulartumors,includingWilm’stumorandmostnephroblastomasandisalsoexertsproangiogenicactivities.
Description
NOVHumanRecombinantproducedinHEK293cellsisasingle,glycosylatedpolypeptidechain(a.a33-357)containing331aminoacidsincludinga6a.aC-terminalHistag.Thetotalmolecularmassis36.5kDa(calculated).
Source
HEK293cells.
PhysicalAppearance
Formulation
NOVfiltered(0.4µm)andlyophilizedfrom0.5mg/mlinPBSand5%(w/v)trehalose.
Solubility
Itisrecommendedtoadd200µldeionizedwatertoprepareaworkingstocksolutionofapproximately0.5mg/mlandletthelyophilizedpelletdissolvecompletely.NOVisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
StABIlity
Storelyophilizedproteinat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcycles.Reconstitutedproteincanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeafteroneweekat4°C.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
QRCPPQCPGRCPATPPTCAPGVRAVLDGCSCCLVCARQRGESCSDLEPCDESSGLYCDRSADPSNQTGICTAVEGDNCVFDGVIYRSGEKFQPSCKFQCTCRDGQIGCVPRCQLDVLLPEPNCPAPRKVEVPGECCEKWICGPDEEDSLGGLTLAAYRPEATLGVEVSDSSVNCIEQTTEWTACSKSCGMGFSTRVTNRNRQCEMLKQTRLCMVRPCEQEPEQPTDKKGKKCLRTKKSLKAIHLQFKNCTSLHTYKPRFCGVCSDGRCCTPHNTKTIQAEFQCSPGQIVKKPVMVIGTCTCHTNCPKNNEAFLQELELKTTRGKMHHHHHH.
SafetyDataSheet
SDS
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theymaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
