Cataloguenumber
CYT-227
Synonyms
OBProtein,ObesityProtein,OBS,Obesityfactor.
Introduction
A16-kDapeptidehormonesecretedfromwhite
ADIpocytesandimplicatedintheregulationoffoodintakeandenergybalance.Leptinprovidesthekeyafferentsignalfromfatcellsinthefeedbacksystemthatcontrolsbodyfatstores.
Description
LeptinRatRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining147aminoacidsandhavingamolecularmassof16kDa.TheLeptinispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutionwith0.0045mMNaHCO3.
Solubility
ItisrecommendedtoreconstitutethelyophilizedLeptininsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedLeptinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionLeptinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbySEC-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeAla-Val-Pro-Ile-His.
BiologicalactivityofLeptinRatisevidencedbyinducingproliferationofBAF/3cellsstablytransfectedwiththelongformofhumanleptinreceptor.
Proteincontent
Proteinquantitationwascarriedoutbytwoindependentmethods:1.UVspectroscopyat280nmusingtheabsorbencyvalueof0.201astheextinctioncoefficientfora0.1%(1mg/ml)solution.ThisvalueiscalculatedbythePCGENEcomputeranalysisprogramofproteinsequences(IntelliGenetics).2.AnalysisbyRP-HPLC,usingacalibratedsolutionofLeptinratasaReferenceStandard.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
SafetyDataSheet
References
1.Title:ExpressionofHypothalamicKiSS-1SystemandRescueofDefectiveResponsesbyKisspeptininStreptozotocin-InducedDiabeticMaleRats.Publication:doi:10.2337/db05-1584DiabetesSeptember2006vol.55no.92602-2610Link:http://diabetes.diabetesjournals.org/content/55/9/2602.full?patientinform-links=yes&legid=diabetes;55/9/26022.Title:Desensitizationofresponsestokisspeptininthefemalerat:analysesofLHandFSHsecretionatdifferentdevelopmentalandmetabolicstates.Publication:AmJPhysiolEndocrinolMetab294:E1088–E1096,2008.294:E1088–E1096,2008.FirstpublishedApril15,2008;doi:10.1152/ajpendo.90240.2008.Link:http://ajpendo.physiology.org/content/294/6/E1088.full.pdf3.Title:p38MAPKandLXR?mediateleptineffectonSREBP-1cexpressioninhepaticstellatecells(shorttitle:LeptineffectonSREBP-1cexpression).Publication:Link:http://www.molmed.org/content/papers%20in%20press/11_243_Yan.pdf4.Title:Neuroprotectionbyleptininaratmodelofpermanentcerebralischemia:effectsonSTAT3phosphorylationindiscretecellsofthebrain.Publication:Citation:CellDeathandDisease(2011)2,e238;doi:10.1038/cddis.2011.125Publishedonline8December2011.Link:http://www.nature.com/cddis/journal/v2/n12/full/cddis2011125a.html
5.Title:ThemammaliantargetofrapamycinasnovelcentralregulatorofpubertyonsetviamodulationofhypothalamicKiss1system.Publication:Endocrinology150.11(2009):5016-5026.Linkhttp://press.endocrine.org/doi/full/10.1210/en.2009-0096