Cataloguenumber
CYT-228
Synonyms
OBProtein,ObesityProtein,OBS,Obesityfactor.
Introduction
A16-kDapeptidehormonesecretedfromwhite
ADIpocytesandimplicatedintheregulationoffoodintakeandenergybalance.Leptinprovidesthekeyafferentsignalfromfatcellsinthefeedbacksystemthatcontrolsbodyfatstores.
Description
LeptinHumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining146aminoacidsandhavingamolecularmassof16kDa.TheLeptinispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutionwith0.0045mMNaHCO3.
Solubility
ThelyophilizedLeptinisverysolubleinwaterandmostaqueousbuffersbelowandabovetheisoelectricpoint.
LyophilizedLeptinalthoughstableatroomtemperature,shouldbestoreddesiccatedbelow0°C.ReconstitutedLeptinisbeststoredrefrigeratedat4°C.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbySEC-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeAla-Val-Pro-Ile-Gln.
BiologicalActivityisevidencedbyinducingproliferationofBAF/3cellsstablytransfectedwiththelongformofhumanleptinreceptor.
Proteincontent
Proteinquantitationwascarriedoutbytwoindependentmethods:1.UVspectroscopyat280nmusingtheabsorbencyvalueof0.878astheextinctioncoefficientfora0.1%(1mg/ml)solution.ThisvalueiscalculatedbythePCGENEcomputeranalysisprogramofproteinsequences(IntelliGenetics).2.AnalysisbyRP-HPLC,usingacalibratedsolutionofLeptinasaReferenceStandard.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
SafetyDataSheet
References
1.Title:LeptinandAmylinActinanAdditiveMannertoActivateOverlappingSignalingPathwaysinPeripheralTissuesInvitroandexvivostudiesinhumans.Publication:PublishedonlinebeforeprintSeptember24,2010,doi:10.2337/dc10-0518DiabetesCareJanuary2011vol.34no.1132-138.Link:http://care.diabetesjournals.org/content/34/1/132.full2.Title:TheMammalianTargetofRapamycinasNovelCentralRegulatorofPubertyOnsetviaModulationofHypothalamicKiss1System.Publication:PublishedonlinebeforeprintSeptember4,2009,doi:10.1210/en.2009-0096EndocrinologyNovember1,2009vol.150no.115016-5026Link:http://endo.endojournals.org/content/150/11/5016.full3.Title:p38Mitogen-ActivatedProteinKinaseandLiverXReceptor-?MediatetheLeptinEffectonSterolRegulatoryElementBindingProtein-1cExpressioninHepaticStellateCells.Publication:MolMed.2012;18(1):10–18.
Publishedonline2011October3.doi:10.2119/molmed.2011.00243Link:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269638/
4.Title:PI3Kintegratestheeffectsofandleptinonlarge-conductanceCa2+-activatedK+channelsinneuropeptideYneuronsofthehypothalamicarcuatenucleus
Publication:AmericanJournalofPhysiology-EndocrinologyandMetabolism298.2(2010):E193-E201.Link:http://ajpendo.physiology.org/content/298/2/E193.shorty