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prospecbio(授权代理)
主营:主营:重组和天然蛋白、细胞因子和生长因子、趋化因子、蛋白激酶、激素和酶、热休克蛋白、病毒抗原、单克隆及多克隆抗体。
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当前位置: 首页 > 产品中心 > Growth_factor > Prospec/Leptin Chicken/Leptin Chicken Recombinant/20µg/CYT-505-20µg-20µg
商品详细Prospec/Leptin Chicken/Leptin Chicken Recombinant/20µg/CYT-505-20µg-20µg
Prospec/Leptin Chicken/Leptin Chicken Recombinant/20µg/CYT-505-20µg-20µg
Prospec/Leptin Chicken/Leptin Chicken Recombinant/20µg/CYT-505-20µg-20µg
商品编号: CYT-505-20µg-20µg
品牌: prospecbio
市场价: ¥1000.00
美元价: 450.00
产地: 美国(厂家直采)
公司:
产品分类: 生长因子
公司分类: Growth_factor
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Cataloguenumber

CYT-505

Synonyms

OBProtein,ObesityProtein,OBS,Obesityfactor.

Introduction

A16-kDapeptidehormonesecretedfromwhiteADIpocytesandimplicatedintheregulationoffoodintakeandenergybalance.Leptinprovidesthekeyafferentsignalfromfatcellsinthefeedbacksystemthatcontrolsbodyfatstores.

Description

LeptinChickenRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining145aminoacidsandhavingamolecularmassof16kDa.TheLeptinispurifiedbyproprietarychromatographictechniques.

Source

EscherichiaColi.

PhysicalAppearance

SterileFilteredWhitelyophilized(freeze-dried)powder.

Formulation

Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutionwith0.0045mMNaHCO3.

Solubility

ItisrecommendedtoreconstitutethelyophilizedLeptininsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.

StABIlity

LyophilizedLeptinalthoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionLeptinshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

Purity

Greaterthan95.0%asdeterminedby:(a)AnalysisbySEC-HPLC.(b)AnalysisbySDS-PAGE.

Aminoacidsequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeAla-Val-Pro-Cys-Gln.

BIOLOGicalActivity

BiologicalactiveasevidencedbyinducingproliferationofBAF/3cellsstablytransfectedwiththelongformofhumanleptinreceptor.Itsactivityishowever5-10foldlowerascomparedtomammalianleptins.

Proteincontent

Proteinquantitationwascarriedoutbytwoindependentmethods1.UVspectroscopyat280nmusingtheabsorbencyvalueof0.19astheextinctioncoefficientfora0.1%(1mg/ml)solution.ThisvalueiscalculatedbythePCGENEcomputeranalysisprogramofproteinsequences(IntelliGenetics).2.AnalysisbyRP-HPLC,usingcalibratedsolutionofLeptinChickenasaReferenceStandard.

SafetyDataSheet

SDS

Usage

Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
品牌介绍
    ProSpec-Tany TechnoGene Ltd.公司创立人Dr. Tanchum (Tany) Amarant 主要致力于蛋白生物化学方面的研究工作,1947年出生于以色列。1991-1997创建ReProGen 公司,从事细胞因子和趋化因子的大量生产及纯化;1997-2000创建ProSpec-TechnoGene,主要生产用于生命科学研究的各种重组蛋白及相关抗体。拥有专利United States Patent 5434135。       ProSpec is an evolving biotech company providing highly purified proteins worldwide which serve the research community with cytokine-related products for cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cell research.       Among our proteins are Interferons, Interleukins, Bone Morphogenic Proteins, Tumor Necrosis factors, Leptins, Stem Cell factors, Prolactins, Chemokines, Antibodies, Peptides, Enzymes, Viral Antigens, various growth factors such as: Platelet Derived, Epidermal, Insulin Like, Nerve, Connective Tissue, Vascular Endothelial, Fibroblast and more.....       Our research team and scientists have been able to assemble more than 1700 recombinant proteins, peptides and antibodies for your research needs. The proteins are rigorously tested to meet the research and development demand for excellent quality, uncompromising biological activity at competitive prices.       ProSpec has established sterling reputation among the scientific community, a reputation built on innovative products, customer service and attention to detail. Our reputation for quality is an essential factor in achieving a favorable position in the market place and the benefits associated with manufacturing top quality products.