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Prospec/IGF1 Human, R36Q/Insulin Like Growth Factor-1, Mutant R36Q Human Recombinant/50µg/CYT-1090-50µg-50µg
Cataloguenumber
CYT-1090
Synonyms
Introduction
Thesomatomedins,orinsulin-likegrowthfactors(IGFs),compriseafamilyofpeptidesthattakespartinmammaliangrowthanddevelopment.IGF1mediatesvariousgrowth-promotingeffectsofgrowthhormone(GH;MIM139250).Earlystudiesshowedthatgrowthhormonedidnotdirectlystimulatetheincorporationofsulfateintocartilage,butratheractedthroughaserumfactor,termed"sulfationfactor,"whichlaterbecameknownas"somatomedin"(Daughadayetal.,1972).3mainsomatomedinshavebeencharacterized:somatomedinC(IGF1),somatomedinA(IGF2;MIM147470),andsomatomedinB(MIM193190)(Rotwein,1986;Rosenfeld,2003).
Description
IGF1R36QHumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining70aminoacidsandhavingamolecularmassofApproximately7.6kDa.
TheIGF1R36Qispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
IGF1R36QLyophilizedfroma0.2µmfilteredconcentratedsolutionin20mMPBand150mMNaCl,pH5.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedIGF1R36Qinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSQRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA.
SafetyDataSheet
SDS