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Prospec/IGF1 Human, A70T/Insulin Like Growth Factor-1, Mutant A70T Human Recombinant/50µg/CYT-1091-50µg-50µg
Cataloguenumber
CYT-1091
Synonyms
Introduction
Thesomatomedins,orinsulin-likegrowthfactors(IGFs),compriseafamilyofpeptidesthattakespartinmammaliangrowthanddevelopment.IGF1mediatesvariousgrowth-promotingeffectsofgrowthhormone(GH;MIM139250).Earlystudiesshowedthatgrowthhormonedidnotdirectlystimulatetheincorporationofsulfateintocartilage,butratheractedthroughaserumfactor,termed"sulfationfactor,"whichlaterbecameknownas"somatomedin"(Daughadayetal.,1972).3mainsomatomedinshavebeencharacterized:somatomedinC(IGF1),somatomedinA(IGF2;MIM147470),andsomatomedinB(MIM193190)(Rotwein,1986;Rosenfeld,2003).
Description
IGF1A70THumanRecombinantproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining70aminoacidsandhavingamolecularmassofApproximately7.7kDa.
TheIGF1A70Tispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
IGF1A70TLyophilizedfroma0.2µmfilteredconcentratedsolutionin20mMPBand150mMNaCl,pH6.0.
Solubility
ItisrecommendedtoreconstitutethelyophilizedIGF1A70Tinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKST.
SafetyDataSheet
SDS