Cataloguenumber
CYT-218
Synonyms
Prostatropin,FGF-basic,BasicFGF,HBGF-2,FGF-2,FGF-b.
Introduction
Basicfibroblastgrowthfactorisamemberofthefibroblastgrowthfactor(FGF)family.FGFfamilymemberspossessbroadmitogenicandcellsurvivalactivities,andareinvolvedinavarietyofBIOLOGicalprocesses,includingembryonicdevelopment,cellgrowth,morphogenesis,tissuerepair,tumorgrowthandinvasion.Thisproteinfunctionsasamodifierofendothelialcellmigrationandproliferation,aswellasanangiogenicfactor.Itactsasamitogenforavarietyofmesoderm-andneuroectoderm-derivedcellsinvitro,thusisthoughttobeinvolvedinorganogenesis.Threealternativelysplicedvariantsencodingdifferentisoformshavebeendescribed.TheHPR-bindinggrowthfactorsareangiogenicagentsinvivoandarepotentmitogensforavarietyofcelltypesinvitro.Therearedifferencesinthetissuedistributionandconcentrationofthese2growthfactors.
Description
FibroblastGrowthFactor-2HumanRecombinant(FGF-2)producedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining154aminoacidsandhavingamolecularmassof17.2kDa.TheFGF-bispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutioninPBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedFibroblastGrowthFactorBasicinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedFibroblastGrowthFactor-2althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFGF-bshouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
Purity
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
AAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS.
BiologicalActivity
TheED50,calculatedbythedose-dependantproliferationofBAF3cellsexpressingFGFreceptors(measuredby3H-thymidineuptake)is<0.5ng/ml,correspondingtoaspecificactivityof2,000,000Units/mg.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.
References
1.Title:ActivationoftheHedgehogpathwayinpilocyticastrocytomasPublication:NeuroOncol(2010)12(8):790-798.doi:10.1093/neuonc/noq026Firstpublishedonline:March11,2010Link:http://neurooncology.oxfordjournals.org/content/12/8/790.full2.Title:Phenotypereversioninfetalhumanliverepithelialcellsidentifiestheroleofanintermediatemeso-endodermalstagebeforehepaticmaturationPublication:AdvanceOnlinePublicationMarch4,2008doi:10.1242/?jcs.019315April1,2008JCellSci121,1002-1013.Link:http://jcs.biologists.org/content/121/7/1002.full3.Title:EmbryonicStemCell-DerivedGlialPrecursorsasaVehicleforSulfamidaseProductionintheMPS-IIIAMouseBrain.Publication:
CellTransplantationISSN:0963-6897DOI:10.3727/096368910X498944Volume19,Issue8,pages985-998Copyright©2010CognizantComm.CorpLink:http://www.ingentaconnect.com/content/cog/ct/2010/00000019/00000008/art000074.Title:AdultHumanDentalPulpStemCellsDifferentiateTowardFunctionallyActiveNeuronsUnderAppropriateEnvironmentalCues.Publication:Articlefirstpublishedonline:22MAY2008DOI:10.1634/stemcells.2007-0979Copyright©2008AlphaMedPress.Link:http://onlinelibrary.wiley.com/doi/10.1634/stemcells.2007-0979/full5.Title:SuccessfulimplantationofphysiologicallyfunctionalbioengineeredmouseinternalanalsphincterPublication:AmericanJournalofPhysiology-GastrointestinalandLiverPhysiology299.2(2010):G430-G439.Link:http://ajpgi.physiology.org/content/299/2/G430