Cataloguenumber
CYT-075
Synonyms
Fibroblastgrowthfactor1,FGF-1,Acidicfibroblastgrowthfactor,aFGF,bindinggrowthfactor1,HBGF-1,Fgf1,Fgfa,HBGF1.
Introduction
Acidicfibroblastgrowthfactorisamemberofthefibroblastgrowthfactor(FGF)family.FGFfamilymemberspossessbroadmitogenicandcellsurvivalactivities,andareinvolvedinavarietyofBIOLOGicalprocesses,includingembryonicdevelopment,cellgrowth,morphogenesis,tissuerepair,tumorgrowthandinvasion.Thisproteinfunctionsasamodifierofendothelialcellmigrationandproliferation,aswellasanangiogenicfactor.Itactsasamitogenforavarietyofmesoderm-andneuroectoderm-derivedcellsinvitro,thusisthoughttobeinvolvedinorganogenesis.Threealternativelysplicedvariantsencodingdifferentisoformshavebeendescribed.Thebindinggrowthfactorsareangiogenicagentsinvivoandarepotentmitogensforavarietyofcelltypesinvitro.Therearedifferencesinthetissuedistributionandconcentrationofthese2growthfactors.
Description
FibroblastGrowthFactor-acidicRatRecombinant(FGF-1)producedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining141aminoacidsandhavingamolecularmassof15.9kDa.TheFGFacidicispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Lyophilizedataconcentrationof1mg/mlin5mMNa2PO4,pH-7.5and50mMNaCl.
Solubility
ItisrecommendedtoreconstitutethelyophilizedFibroblastGrowthFactor-acidicinsterile18MΩ-cmH2Onotlessthan100µg/ml,whichcanthenbefurtherdilutedtootheraqueoussolutions.
LyophilizedFibroblastGrowthFactor-1althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFGF-ashouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan98.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MFNLPLGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESAGEVYIKGTETGQYLAMDTEGLLYGSQTPNEECLFLERLEENHYNTYTSKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD.
BiologicalActivity
TheED50asdeterminedbythedose-dependentproliferationofmouseBALB/c3T3cells,islessthan0.2ng/mlcorrespondingtoaSpecificActivityof5x106IU/mg.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.