Cataloguenumber
CYT-264
Synonyms
HBGF-1,ECGF-beta,FIBP,FGFIBP,FIBP-1,ECGF,ECGFA,GLIO703,FGF1,FGF-a.
Introduction
Acidicfibroblastgrowthfactorisamemberofthefibroblastgrowthfactor(FGF)family.FGFfamilymemberspossessbroadmitogenicandcellsurvivalactivities,andareinvolvedinavarietyofBIOLOGicalprocesses,includingembryonicdevelopment,cellgrowth,morphogenesis,tissuerepair,tumorgrowthandinvasion.Thisproteinfunctionsasamodifierofendothelialcellmigrationandproliferation,aswellasanangiogenicfactor.Itactsasamitogenforavarietyofmesoderm-andneuroectoderm-derivedcellsinvitro,thusisthoughttobeinvolvedinorganogenesis.Threealternativelysplicedvariantsencodingdifferentisoformshavebeendescribed.Thehep-bindinggrowthfactorsareangiogenicagentsinvivoandarepotentmitogensforavarietyofcelltypesinvitro.Therearedifferencesinthetissuedistributionandconcentrationofthese2growthfactors.
Description
FibroblastGrowthFactor-acidicHumanRecombinant(FGF-1)producedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining140aminoacidsandhavingamolecularmassofapproximately15.8kDa.TheFGFacidicispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)sterilesolutioncontainingPBS,pH7.4.
Solubility
ItisrecommendedtoreconstitutethelyophilizedFibroblastGrowthFactor-acidicinsterile18MΩ-cmH2Oat4degreesCelsiusataconcentrationof0.1mg-0.25mgper1ml.Allowsampletositfor5min.at4degrees,spintoremoveprecipitant.
LyophilizedFibroblastGrowthFactor-1althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionFGF-ashouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.Pleasepreventfreeze-thawcycles.
Purity
Greaterthan95.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
Aminoacidsequence
MFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD.
BiologicalActivity
TheED50,calculatedbythedose-dependantproliferationofmouseBALB/c3T3cellsis<0.5ng/ml,correspondingtoaspecificactivityof>2,000,000IU/mg.
SafetyDataSheet
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.