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Prospec/EPGN Human, Sf9/Epigen Human Recombinant, Sf9/10µg/CYT-1038-10µg-10µg
Cataloguenumber
CYT-1038
Synonyms
Epithelialmitogen,EPG,EpithelialMitogenHomolog(Mouse),EpithelialMitogenHomolog,ALGV3072,PRO9904,Epigen,EPGN.
Introduction
EPGNisanEGF-relatedpolypeptidegrowthfactorthatsignalsthroughtheErbBreceptor-1.EPGNisproducedinnumeroustissues,includingthetestis,liver,heartandincertaintumorcells.EPGNismitogenicforfibroblastsandepithelialcells.HumanEPGNisoriginallysynthesizedasaglycosylated14.7kDatransmembraneprecursorprotein,whichisprocessedbyproteolyticcleavagetoproduceamaturesolublesequence.
Description
EPGNproducedinSf9Insectcellsisasingle,glycosylatedpolypeptidechaincontaining97aminoacids(23-110a.a.)andhavingamolecularmassof10.8kDa(MolecularsizeonSDS-PAGEwillappearatapproximately13.5-18kDa).EPGNisexpressedwithan9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
EPGNproteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.Store,frozenat-20°Cforlongerperiodsoftime.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPAAVTVTPPITAQQGNWTVNKTEADNIEGPIALKFSHLCLEDHNSYCINGACAFHHELEKAICRCFTGYTGERCEHLTLTSYAVDSYEKHHHHHH.
SafetyDataSheet
SDS