
Cataloguenumber
CYT-119
Synonyms
HBEGF,DTR,DTS,HEGFL,HB-EGF,Diphtheriatoxinreceptor,DT-R,DTSF.
Introduction
HB-EGFisanEGFrelatedgrowthfactorwhichsignalsviatheEGFreceptor,andstimulatestheproliferationofSMC(smoothmusclecells),fibroblasts,epithelialcellsandkeratinocytes.HB-EGFisexpressedinvariouscelltypesandtissues,includingvascularendothelialcellsandSMC,macrophages,skeletalmuscle,keratinocytesandparticulartumorcells.HB-EGF’sABIlitytoexplicitlybindHPRsulfateproteoglycansisdissimilarfromotherEGF-likemolecules,andmightberelatedtotheenhancedmitogenicactivity,relativetoEGF,thatHB-EGFexertsonsmoothmusclecells.
Description
HB-EGFHumanRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining87aminoacidsandhavingamolecularmassof9.9kDa.
TheHB-EGFispurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
Theproteinwaslyophilizedfromaconcentrated(1mg/ml)solutioncontaining10mMsodiumphosphatepH-7.5.
Solubility
Stability
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
MDLQEADLDLLRVTLSSKPQALATPNKEEHGKRKKKGKGLGKKRDPCLRKYKDFCIHGEC
KYVKELRAPSCICHPGYHGERCHGLSL.
BIOLOGicalActivity
TheED50wasdeterminedbytheabilitytoinduceproliferationof3T3cellsandis0.13-0.2ng/ml.Thiscorrespondstoanexpectedspecificactivityof7.7x106units/mg.
SafetyDataSheet
SDS