Cataloguenumber
CYT-1073
Synonyms
Tumornecrosisfactorreceptorsuperfamilymember21,BM-018,CD358,DR6,Deathreceptor6.
Introduction
Tumornecrosisfactorreceptorsuperfamilymember21orTNFRSF21,isaproteinthatlocatedinthecellmembranefromtheTNFreceptorsuperfamily.ByactivatingtheNF-kappaBpathway,TNFRSF21promotescellapoptosis.Thedegenerationofcellscausedbyactivatingcaspase3andcaspase6,theTNFRSF21bindstotheN-terminalAPPinneuronalcellbodiesandaxonswhichleadstoapoptosis.TNFRSF21takespartinsignalingcascadesactivatedbystimulationofT-cellreceptor.
Description
TNFRSF21producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining547aminoacids(42-349a.a.)andhavingamolecularmassof60.4kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).TNFRSF21isexpressedwitha239aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
TNFRSF21proteinsolution(1mg/ml)containsphosphatebufferedsaline(pH7.4),10%glyceroland0.1mMPMSF.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
QPEQKASNLIGTYRHVDRATGQVLTCDKCPAGTYVSEHCTNTSLRVCSSCPVGTFTRHENGIEKCHDCSQPCPWPMIEKLPCAALTDRECTCPPGMFQSNATCAPHTVCPVGWGVRKKGTETEDVRCKQCARGTFSDVPSSVMKCKAYTDCLSQNLVVIKPGTKETDNVCGTLPSFSSSTSPSPGTAIFPRPEHMETHEVPSSTYVPKGMNSTESNSSASVRPKVLSSIQEGTVPDNTSSARGKEDVNKTLPNLQVVNHQQGPHHRHILKLLPSMEATGGEKSSTPIKGPKRGHPRQNLHKHFDINEHLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
SafetyDataSheet
SDS
