Cataloguenumber
CYT-1055
Synonyms
TNFRSF10B,CD262,DR5,KILLER,KILLER/DR5,TRAIL-R2,TRAILR2,TRICK2,TRICK2A,TRICK2B,TRICKB,ZTNFR9,Deathreceptor5,TNF-relatedapoptosis-inducingligandreceptor2.
Introduction
TRAILReceptor-1(DR4)andTRAILReceptor-2(DR5)aremembersoftheTNFRsuperfamilyoftransmembraneproteinsandcontainacytoplasmic"deathdomain",whichiscapableofactivatingthecell"sapoptoticmachinery.ThesereceptorsareactivatedbybindingtoeithermembraneanchoredorsolubleTRAIL/Apo2L.
Description
TNFRSF10BproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining394aminoacids(56-210a.a.)andhavingamolecularmassof43.9kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately40-57kDa).TNFRSF10Bisexpressedwitha239aminoacidhIgG-HistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Insectcells.
PhysicalAppearance
Formulation
TNFRSF10Bproteinsolution(1mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
MeasuredbyitsabilitytoinhibitcytotoxicityassayusingJurkathumanTlymphocyte.TheED50forthiseffect≤to5ng/mlwithTRAIL
.
Aminoacidsequence
ITQQDLAPQQRAAPQQKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCEEGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKESGTKHSGEVPAVEETVTSSPGTPASPCSLEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH.
SafetyDataSheet
SDS