
Cataloguenumber
CYT-1077
Synonyms
IL6R-alpha,CD126,IL-6R1,CD126antigen,IL6RA,Interleukin6receptor,Interleukin6receptoralphasubunit,Interleukin-6receptoralphachainprecursor,Bcellstimulatoryfactor-2,Membraneglycoprotein80,gp80,IL-6R,MGC104991.
Introduction
TheIL-6receptorcomplexiscomposedoftwomembraneglycoproteins:thelowaffinityreceptorandthesignaltransducingcomponent.ThesolubleformofIL6RisfoundintheurineofhealthyadulthumansandtheserumofHIVpositiveindividuals,aswellasinthecellculturesupernatantsofstimulatedPBMC’s.ThissolubleformofIL6Rresultsfromeitherproteolyticcleavagefromthemembrane,oranisoformderivedsplicevariant.
Description
sIL6RproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechain(20-365a.a.)andfusedtoa9aaHisTagatC-terminuscontainingatotalof355aminoacidsandhavingamolecularmassof39.6kDa.sIL6Rshowsmultiplebandsbetween40-57kDaonSDS-PAGE,reducingconditionsandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
sIL6Rproteinsolution(0.25mg/ml)contains10%glycerol&Phosphatebufferedsaline(pH7.4).
StABIlity
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPLAPRRCPAQEVARGVLTSLPGDSVTLTCPGVEPEDNATVHWVLRKPAAGSHPSRWAGMGRRLLLRSVQLHDSGNYSCYRAGRPAGTVHLLVDVPPEEPQLSCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGCGILQPDPPANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWMVKDLQHHCVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSPPAENEVSTPMQALTTNKDDDNILFRDSANATSLPVQDSSSVPLPHHHHHH
SafetyDataSheet
SDS