
Cataloguenumber
CYT-1048
Synonyms
IL6,Ifnb2,ILg6.
Introduction
Interleukin-6isapotentpro-inflammatorycytokineprimarilyproducedbyactivatedTcellsandanassortmentofothercellsincludingendothelialcellsandmacrophages.IL-6affectsBandTlymphocytesandhasbeenshowntohavearoleinhostdefense,acutephasereactions,immuneresponsesandhematopoiesis.
Description
IL6producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining196aminoacids(25-211a.a.)andhavingamolecularmassof22.8kDa(MolecularsizeonSDS-PAGEwillappearatapproximately18-28kDa).IL6isexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
IL6proteinsolution(0.5mg/ml)contains20%glycerol&PhosphateBufferedSaline(pH7.4).
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
MeasuredinacellproliferationassayusingM-NFS-60mouseBcell.TheED50forthiseffectsislessorequalto0.1ng/ml.
SafetyDataSheet
SDS
Aminoacidsequence
ADPFPTSQVRRGDFTEDTTHNRPVYTTSQVGGLITYVLREILEMRKELCNGNSDCMNSDDALSENNLKLPEIQRNDGCFQTGYNQEICLLKICSGLLEFRFYLEFVKNNLQDNKKDKARVIQSNTETLVHIFKQEIKDSYKIVLPTPTSNALLMEKLESQKEWLRTKTIQLILKALEEFLKVTMRSTRQTHHHHHH