
Cataloguenumber
CYT-1086
Synonyms
Interleukin2receptorsubunitbeta,IL2RB,CD122,IL15RB,P70-75,IL-2Rsubunitbeta.
Introduction
Interleukin2receptorisaproteinthatinvolvesimmuneresponsesthataremediatedthroughTcells.TheIL2receptorexistsinthreewaysinwhichitcanbindtoIL2.CD25isthelowestaffinity(amonomerofthealphasubunit),therefore,itisnotpartofthesignaltransductionprocess.Thehighestaffinityhasthreeheterotrimersubunits(alpha/beta/gamma),theintermediateaffinityisfoundtohavegamma/betasubunitheterodimer.BothformsarepartofthereceptormediatedendocytosisandmitogenicsignalstransductionfromIL2.
Description
IL2RBproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechain(27-240a.a.)andfusedtoa6aaHisTagatC-terminuscontainingatotalof220aminoacidsandhavingamolecularmassof25.5kDa.IL2RBshowsmultiplebandsbetween28-40kDaonSDS-PAGE,reducingconditionsandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
IL2RBproteinsolution(0.25mg/ml)containsPhosphateBufferedSaline(pH7.4)&10%glycerol.
StABIlity
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
AVNGTSQFTCFYNSRANISCVWSQDGALQDTSCQVHAWPDRRRWNQTCELLPVSQASWACNLILGAPDSQKLTTVDIVTLRVLCREGVRWRVMAIQDFKPFENLRLMAPISLQVVHVETHRCNISWEISQASHYFERHLEFEARTLSPGHTWEEAPLLTLKQKQEWICLETLTPDTQYEFQVRVKPLQGEFTTWSPWSQPLAFRTKPAALGKDTHHHHHH.
SafetyDataSheet
SDS