
Cataloguenumber
CYT-1072
Synonyms
Interleukin-21isoform1,CVID11,IL-21,Za11.
Introduction
Interleukin-21isoform1orIL21,categorizedasaThelpercytokine.IL21ispartoftheregulationofimmuneresponses,haematopoiesisandcell-mediatedresponses.IL21hasakeyroleinhaematopoiesisandimmuneresponsesregulation,thereforecanaffectlymphocytedevelopment.ActivationofTcellsleadstoproductionoftheIL21.Theproteinhasacrucialroleintheviralinfectionspersistence.
Description
IL21producedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining145aminoacids(30-162a.a.)andhavingamolecularmassof16.9kDa.(MolecularsizeonSDS-PAGEwillappearatapproximately18-28kDa).IL21isexpressedwitha12aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
Sterilefilteredcolorlesssolution.
Formulation
IL21proteinsolution(0.25mg/ml)containsphosphatebufferedsaline(pH7.4)and20%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan85.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPEFMQGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDSHHHHHH.
SafetyDataSheet
SDS