
Cataloguenumber
CYT-1096
Synonyms
Interleukin-2,IL-2,T-cellgrowthfactor,TCGF.
Introduction
IL2isasecretedcytokinethatisessentialfortheproliferationofTandBlymphocytes.Thereceptorofthiscytokineisaheterotrimericproteincomplexwhosegammachainisalsosharedbyinterleukin4andinterleukin7.TheexpressionofIL2inmaturethymocytesismonoallelic,whichrepresentsanunusualregulatorymodeforcontrollingtheexactexpressionofasinglegene.
Description
Interleukin-2CanineRecombinantproducedinE.coliisanon-glycosylatedmonomerchaincontaining136aminoacidsandhavingamolecularmassof15.6kDa.
IL-2ispurifiedbyproprietarychromatographictechniques.
Source
EscherichiaColi.
PhysicalAppearance
Formulation
Theproteinwaslyophilizedfromasterile(0.2µm)filteredsolutioncontaining20mMsodiumbicarbonate,pH8.5.
Solubility
ItisrecommendedtoreconstitutethelyophilizedIL-2inSterile10mMsodiumbicarbonate,pH8.5at0.1mg/mL,whichcanthenbefurtherdilutedtootheraqueoussolutions.
StABIlity
LyophilizedIL-2althoughstableatroomtemperaturefor3weeks,shouldbestoreddesiccatedbelow-18°C.UponreconstitutionIL-2shouldbestoredat4°Cbetween2-7daysandforfutureusebelow-18°C.
Pleasepreventfreeze-thawcycles.
Aminoacidsequence
MAPITSSSTKETEQQMEQLLLDLQLLLNGVNNYENPQLSRMLTFKFYTPKKATEFTHLQCLAEELKNLEEVLGLPQSKNVHLTDTKELISNMNVTLLKLKGSETSYNCEYDDETATITEFLNKWITFSQSIFSTLT.
Purity
Greaterthan95.0%asdeterminedbySDS-PAGE.
BIOLOGicalActivity
TheED50,asdeterminedbyCTLL-2cellproliferationis<5ng/mlcorrespondingtoaspecificactivitywhichis≥2.0x10^5units/mg.
SafetyDataSheet
SDS