
Cataloguenumber
CYT-1062
Synonyms
Il1a,Il-1a,Hematopoietin-1,Lymphocyte-activatingfactor(LAF),EndogenousPyrogen(EP),LeukocyteEndogenousMediator(LEM),MononuclearCellFactor(MCF),IL-1alpha,IL1,IL-1A,IL1F1.
Introduction
ThecytokineIL-1alphaorhematopoietin1isamembertotheinterleukin1family,encodedbytheIL1Ageneinhumans.EssentiallyIl-1isinchargeofinflammationandtherisingoffeverandsepsis.Inordertodisturbthementionedprocessesandtreatmentfordiseases,inhibitorsforInterleukin1alphaarebeingdeveloped.NeutrophilsandmacrophagesarethemainactivatorsofIL-1alpha,aswellasendothelialandepithelialcells.Bybindingtotheil1receptoritisamajorpartintheimmuneresponseregulation.Intheactivationprocessoftumornecrosisfactor-alphatheIL1Ahasapartaswell,andhasphysiological,metabolicandhematopoieticactivities.
Description
IL1AMouseRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining165aminoacids(115-270a.a.)andhavingamolecularmassof19.0kDa(MolecularsizeonSDS-PAGEwillappearatapproximately18-28kDa).IL1Aisexpressedwitha9aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
PhysicalAppearance
Formulation
IL1Aproteinsolution(0.5mg/ml)containsPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Purity
Greaterthan90%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPSAPYTYQSDLRYKLMKLVRQKFVMNDSLNQTIYQDVDKHYLSTTWLNDLQQEVKFDMYAYSSGGDDSKYPVTLKISDSQLFVSAQGEDQPVLLKELPETPKLITGSETDLIFFWKSINSKNYFTSAAYPELFIATKEQSRVHLARGLPSMTDFQISHHHHHH.
SafetyDataSheet
SDS